hGeCKO Library A#1 in Hela

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hGeCKO Library A#1 in Hela

Human CRISPR Knockout Pooled Library A(1 vector system)

Product NamehGeCKO Library A#1 in Hela

Cell LineHeLa

Catalog# LIBR-H005A-C300 LIBR-H005A-C500

Size(Applicable Cell Coverage) 300x 500x

Instruction

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Ubigene's CRISPR Library Cell Pool is conducted by utilizing CRISPR iScreen™. Ubigene's CRISPR Library Cell Pool is standardized constructed in batches by firstly obtaining Library Plasmid with high coverage and good uniformity using self-developed high-efficiency competent cells, then packaging the virus using Lentiviral Packaging Kit (#YK-LVP-20) to obtain high-titer CRISPR Library Virus, finally through the exclusive cell pool preparation process, infecting the target cell line with low infect MOI to restrict one virus per cell. Ubigene's CRISPR Library Cell Pools have small batch differences and good reproducibility. Screening on Cell Pools under different pressure conditions can screen target genes suitable for research in different fields.
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Product Information

Library Name
Human CRISPR Knockout Pooled Library A(1 vector system)
Product Name
hGeCKO Library A#1 in Hela
Cell line
HeLa
Organism
Human
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puromycin 1.5µg/ml
Culture medium
90%DMEM+10%FBS
Targeted Genes
19050
gRNA Number
65383
Non-targeting gRNA Number
1000
Cell Image
Cell Reception
EZ-editor™ CRISPR Library Cell Pool will be transported in the form of cryopreserved cells. Upon received, immediately transfer to liquid nitrogen for storage or store briefly(24H) at-80°C freezer, or proceed directly to cell thawing.
CRISPR iScreen™ Product Strength
  • 35+ Libraries
    100+ Cas9 cell lines for screening
    35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
    1
  • Plasmid
    Coverage>99%, uniformity<10
    The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
    2
  • Cell Pool
    Coverage rate up to 99%
    Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
    3
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